| Summary: | 碩士 === 靜宜大學 === 應用化學系 === 82 === Since metals and their complexes play an important role in both
biological chemistry and in enviromental chemistry, the
analysis of metals is rather important. In recent years, high
performance liquid chromatography coupled with various
detectors has become one of the best methods for metal analysis.
In this study, we used 4-（2-pyridylazo）resorcinol (PAR） as a
pre-column reagent, Cu-PAR and Co-PAR were separated and
quantitatively determined by reverse phase high performance
liquid chromatography with UV-VIS detector. We also
investigated the absorption and chromatographic characteristics
of these metal chelates. The optimum condition established in
this work was:acetonitrile/methanol /tartrate buffer (pH=6.5)=
6/30/64 including 1.0×10▋▋ ion-pair reagent(
tetrabutylammonium bromide) The separation was done with C▋▋
eversed phase column. The proposed method was appilied to
determine Cu and Co in a relatively complex substance, namely,
nickel-base superalloy. The precision and accuracy of the
proposed chromatographic method were evaluated。
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