| Summary: | 碩士 === 國立臺灣大學 === 獸醫學系 === 82 === To develop a sensitive diagostic reagent against infection
of mouse hepatitis virus (MHV), one of the most commonly
occurring pathogen in laboratory mouse colonies, hybridoma
cell lines producing monoclonal antibodies to a Taiwanese
strain of MHV were established. Liver emulsion of this local
isolated MHV was used as immunogen to infect 1 to 3 day old
mice intraperitoneally. By indirect immunofluoresence (IF),
monoclonal antibodies reacted in the cytoplasma of infected
cells were identified. Among the 62 IF postive clone, antigenic
specificity were further recognized by
radioimmunoprecipitation and three kinds of viral
structral protein, spike (31 clones), matrix (3 clones) and
nucleocapsid (8 clones), were confirmed. All these antibodies
belong to various IgG subclasses, e.g., IgG1, IgG2a, IgG2b, as
determined by ELISA. Plaque reduction tests were done and 4
clones shown positive to neutralize MHV Taiwanese strain.
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