A Survey of Swine Rotavirus Infections in Taiwan

• Main Authors: Huang, Yu-Jer, 黃于哲
• Other Authors: Lai, Shiow-suey
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/38567696170328612130

碩士 === 國立臺灣大學 === 獸醫學系 === 82 === Reverse transcriptase / polymerase chain reaction (RT/PCR ) was used to detect rotavirus in porcine feces collected from 3 pig farms located in Northern, Middle and Southern part of Taiwan, respectively. The fecal samples were collected from sows, boars, suckling, weaned and fattenning pigs, respectively. Serum samples from sows were also titrated for antibodies to rotavirus by Enzyme - Linked Immunosorbent Assay (ELISA). Type - specific primers were used to serotype OSU and Gottfried strains of group A rotavirus. In this experiment, a total of 1532 fecal samples were examined by RT/PCR： 598 samples(39%) were positive. Among 598 positive samples, 581 were type 5 (OSU)； 5 were type 4 (Gottfried)； 2 samples had mixed infection with OSU and Gottfried strain. However, 14 samples could not be typed. The situation of rotavirus infection at 3 farms was fairly similar. The percentages of positive rate found in pig farms located in Northern, Middle and Southern parts of Taiwan were 37.8%, 41.9% and 37.2% , respectively. Results of rotavirus infection in different stages of pigs showed that the weaned pigs had the highest rate(65.5%), followed by boars(48.6%) and suckling piglets(47.8%). Sows had the lowest rate of infection(22.8%). A total of 153 serum samples of sows were all positive for antibody against rotavirus. The ELISA antibody titers ranged from 1:16 to 1:4096. The sensitivities of RT/PCR and EM to detect rotavirus in fecal samples was compared and discussed.