| Summary: | 碩士 === 國立臺灣大學 === 病理學研究所 === 82 === Nasopharyngeal carcinoma (NPC) is unique among
epithelial maligancies because of its epidemiological
and biological characters. The incidence of NPC in
Chinese living in sourth China, Hong Kong, Taiwan and
Singapore is about 50 to 100 times higher than that in
Caucasians living in the European and American
continents. Clinically, in the late stage of NPC
patients most Chemotherapy drug are ineffective, probably due
to overexpression of mdr-1 gene. This hypothesis has not been
proved yet. So, I wanted to evaluate the status of mdr-1 gene
in our NPC cell lines (a) to identify the P-glycoprotein,
m-RNA and DNA sequence of mdr-1 gene in our NPC cell lines
in both early and late passages, (b) to investigate the
relationship of mdr-1 and p53 gene expression, (c) some
cell lines will be treated with Chemotherapy drugs, such as
Vincristine, VP-16, and then check their mdr-1 and p53 gene's
expression. Approached methods were as following: Southern
blot analysis, Northern blot analysis, in situ RT-PCR
hybridization, immunoelectron microscopy, and
immunohistochemistry. The result of Southern blot
analysis indicates that there is mdr-1 gene
deletion after chemotherapeutic drug treatment. Transcripts
of mdr-1 gene cannot be detected in NPC cell, before and
after chemotherapeutic drug treatment by Northern blot
analysis, but can be detected by in situ RT-PCR hybridization.
Normal transcripts of p53 gene can be detected in two
NPC cell lines both before and after chemotherapeutic
drug treatment. The immunohistochemical findi ngs indicated
that Pg-170 is expressed in NPC cells even stronger before than
after the drug treatment, suggesting that Pg-170 does not
overexpressed in NPC cells in response to drug treatment.
|
|---|
