| Summary: | 碩士 === 國立臺灣大學 === 生理學研究所 === 82 === DNA synthesis and cell activity in deciduomal cells of
the pseudopregnant (PSP) hamsters were investigated using
BrdU (5- bromo-2'-deoxyuridine) incorporation and EIA, and
MTT (3-(4,5)- dimethylthiazol-2-yl-2,5-diphenyltetrazolium
bromide) colormetric assay respectively. PSP was induced in
female hamsters by mating with vasectomized male hamsters at
estrus.Decidualization of one uterine horn in response to
artificial stimuli was induced in hamster on the fourth day
of PSP. Hamsters were decapitated at 3:00 p.m. and 7:00 p.m.
on the fourth day, and at 9:00 a.m. to 10:00 a.m. from
the fifth to the tenth day of PSP. The mesometrial(MS)
and antimesometrial(AMS) deciduoma were separated and cell
suspensions were obtained following trypsin and
collagenase treatment. The incorporation of BrdU into
replicating DNA was monitored as a parameter for DNA
synthesis and was measured using a monoclonal antibody
against BrdU and EIA. The cell bioactivity was detected
using the MTT colormetric assay. Uterine weight rose after
the induction of decidualization and reached the maximum, which
was 15 times of control, on the ninth day of PSP. The
results showed that there were different developmental
patterns of DNA synthesis and cell bioactivity during
decidualization in PSP hamster. The deciduomal DNA
synthesis activity was elevated sharply 10 hr after
stimulation; and the maximum activity was reached on the fifth
day of PSP. The DNA synthesis activity declined
gradually thereafter. The deciduomal cell bioactivity did
not change at the early stage of decidualization. It remained
low till the ninth day of PSP. No difference of DNA
synthesis activity and cell bioactivity were detected
between the MS and AMS deciduoma. The decrease of DNA
synthesis activity and cell bioactivity may lead to
deciduomal regression.
|
|---|
