Biochemical Characterization of the Mouse Seminal Vesicles Autoantigen (SVA)

• Main Authors: Luo Ching-wei, 羅清維
• Other Authors: Chen Yee-Hsiung
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/80056083017870657226

碩士 === 國立臺灣大學 === 生化科學研究所 === 82 === Mouse seminal vesicle autotigen (SVA) has been identified recently from our laborary. It is a 19 kDa glycoprotein and the primary structure of tis protein core has been established by cDNA cloning and proyein sequening. The main task fo this work is to study the structure and the function of SVA. The protein was estimated to contain 25 % beta-structure and no helical structure on the basis of its CD. The complex formation of SVA and zinc ion caused no change in the protein conformation. The carbohydrate moeity of SVA composed of galactose, (L)-fucose, mannose,glucose,N-acetylglucosamine,NGNA, NANA. The results of lectin-glycoprotein agglutination test suggested galactose,(L)- fucose as terminal residues of the conjugated carbohydrate. SVA wae not detectable with antiserum angainst phosphoserine, phosphothreonine ,phosphotyrosine ,nor was phosphorylated by protein kinase C and endogeneouskinase. This revealed that SVA is not a phosphoprotein.The results of immunolocation and Western blotting showed that SVA could bind specifically on the midpiece of sperm.