| Summary: | 碩士 === 國立師範大學 === 生物學研究所 === 82 === Observing the replication of human papillomavirus is difficult
because of lacking an in vitro system to support a stable
episomal viral DNA replication. In 1980, Dr.C. P. Hu established
a cervical carcinoma cell line CC7T, which contains both episomal
and integrated HPV 16 DNA. Compare the DNA sequences with the
prototype HPV 16 DNA, there are point mutations in both E1 andE7
ORFs in epi some, and these two genes are responsible for viral
DNA replication. In order to prove whether these mutations could
effect the existence of episomal DNA in CC7T, we transfected the
cloned episome (pCC7T-BS) and the prototype HPV 16 DNA (pHPV-BS)
into re lated cervical carcinoma and larynx carcinoma cell lines,
HeLa and Hep-2, respectively. Our results showed that there were
neither apparent changes in morphology nor cell death in
transfected cells except pCC7T-BS transfected cell line HeLa. In
HeLa ， t wo days after transfection with pCC7T-BS ， the growth
of the cells delayed and the cells appeared to die 。 On the
other hand ， the replication patterns of both pCC7T-BS and
pHPV-BS were similiar in HeLa and Hep-2. The amount of DNA were
papillomavirus replication reduced gradually a Most of the
informations about nd there were no apparent DNA replication in
both cell lines. It indicated that the mutations occured in
episome might not as important as we expected. come from
researches of the bovine papillomavirus type 1.
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