| Summary: | 碩士 === 國立清華大學 === 輻射生物研究所 === 82 === The cell-killing effects and its possible mechanism of
berberine used alone or in combination with argon ion
laser against 9L rat glioma cells were studied. The survival
fraction of 9L cells after single treatment of
berberine has been investigated by both colony formation
and microtitration (MTT) methods, the half lethal
concentration (LC50) of berberine has been estimated to be 55
μg/ml. After the addition of low energy laser treatment, the
LC50 of berberine markedly decreased to 15μg/ml. The effect
of berberine on biosynthesis for DNA, RNA and protein of 9L
cell was enhanced by argon ion laser. Moreover, when
morphologic change was examined, the 9L cells showed
cytolysis, vacuolization and degeneration after
berberine treatment; the laser treatment enhanced the cell-
killing effect. The study proves the cell-killing effect of
berberine combined with laser treatment on 9L rat
glioma cell line, hence introducing the possibility of
berberine as a photosensitive agent.
|
|---|
