Absorption and metabolism of dietary L-[1-13C]methionine by Penaeus monodon

• Main Authors: Chou, Wei Jung, 周偉融
• Other Authors: Chen, Houng Yung
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/83155223777470088141

碩士 === 國立中山大學 === 海洋生物研究所 === 82 === The present research investigated the absorption and metabolism of L-[1-13C]methionine in Penaeus monodon to understand the reasons why the prawn can not efficiently utilize crystalline amino acids supplemented in diet.Two experiments were conducted. The first experiment studied the change of L-[1-13C] methionine concentration in the hemolymph of Penaeus monodon (20- 30g) within 20 hours after being forced-fed crystalline L- [1-13C] methionine or cellulose acetate phthalate microencapsulated L-[1- 13C] methionine(CAP-L-[1-13 C]methionine). The second experiment examined the deposition and metabolism of L-[1-13C]methionine in the intestine, Hepatopancreas, muscle, and remaining tissues of Penaeus monodon (20-30g)within 6 days after being force-fed crystalline L-[1-13C]methionine or CAP-L-[1-13C]methioine.Penaeus monodon absorbed efficiently either crystalline-L-[1-13C] methionine or CAP-L-[1-13C]methionine. No signfifcant difference in the absorption of CAP-L-[1-13C]methionine or crystalline-L-[1- 13 C]methionine was detected. Almost all the crystalline-L-[1-13 C] and CAP-L-[1-13C]methionine force-fed as absorpted by Penaeus monodon within the first 2 hours and was transported promptly to muscle and remaining tissues. Once in muscle,L-[1-13 C] methionine were not metabolized until 10 hours after L-[1-13 C] methionine deposition in Penaeus monodon fed CAP-L-[1-13C] methionine wasn't significantly higher than that fed crystalline -L-[1-13C]methionine. The present results do not support the two assertion that crystalline amino acids are rapidly absorb and metabolize or the prawn are poor in absorption of crystalline amino acids.Resutls inclined to suggest that leaching of crystalline amino acids during feeding may be the main reason why prawn can not use dietary crystalline amino acid effectively.