| Summary: | 碩士 === 國立成功大學 === 微生物及免役學研究所 === 82 === The standard protocol of immunization (MBP/CFA plus B. pertusis
treatment) can not induce EAE in most strains of mice except
SJL, PL/J or (STL×PC/J) F1 mice. We have tried several
approachs including additional i.p., i.v., i.c. injection to
induce EAE in resistant B6 mice. We found that only combination
of i.p. and i.c. injection could induce EAE in B6 mice. B6 mice
were sensitized by standard procedure. Twenty days later, they
were given additional MBP/CFA i.p.. 2 day later followed by
another i.c. injection of MBP in normal saline. The mice will
develop clinical EAE symptoms (limp tail, hindleg weakness,..)
begining at day 24. The sympton will last several days.
Sometime, the mice will die. If they recover, there were also
some residual neural complication. The histological examination
can observe blood cell infiltration into meninges and
parenchyma. Single i.p. or i.c. injection did not induce
clinical EAE sympton nor histological cell infiltration. The
combination of additional i.p. and i.c. injection can also
induce EAE in other resistant strains such as A.BY. RIII ...
mice. The i.p. injection needs the presence of CFA suggesting
the additional activation of autoreactive lymphocytes. The
requirement of MBP in i.c. injection demonstrate the MBP
specificity of EAE and indicate the recruitment of MBP-specific
autoreactive T cell into the brain that across the BBB. It was
reported that deep cervical lymph nodes (cLN) are drainage
lymph node of brain and C.S.F.. We compared the cell phenotype
and MBP- or anti-CD3-activation between cLN and dLN (inguinal
and axillary). The FACS analysis of cLN cell phenotype also
found major changes during the development of EAE. The kinetic
analysis suggests that Vb8.1.8.2+, CD4+ and Vb8.1.8.2+, CD4-,
CD8- cells are involved and correlated with the development of
EAE. The expression of VLA4+ lymphocyte in cLN is lower than
that of dLN.
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