| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 82 === Pseudorabies virus (PRV), a member of Alphaherpes- virinae, is
the causative agent of pseudorabies (or Aujeszky's disease).
There are at least eight glyco- protein species in PRV. Among
these glycoproteins, the glycoprotein H (gH) is the one which
was found right before glycoprotein L. It is an essential
glycoprotein for virus replication and a protein which induces
neutralizing antibody. In this thesis, the nucleotide sequence
of gH gene of Taiwan PRV strain, TNL strain, were determined.
At first, the putative gH gene was found in the BamHI 11 and 17
fragments of TNL strain as determined by DNA sequencing and
southern hybridization. The complete gH gene was cloned by
ligation of these two fragments. The resulting clone was used
for subsequent subcloning and transcriptional analysis. The gH
gene contains 2,058 bps. Two conserved promoter sequence,
containing a TATA box and a CAT box, was found upstream of the
gene. A potential polyadenylation signal (5'-AATAAA-3) was
found at 3' end of the gene. The gene encodes a protein of 686
amino acid long with a molecular weight of 72,000. The gH
protein possesses features of a transmembrane glycoprotein.
Comparisons of gH protein sequences were made between TNL
strain and two published foreign strains, Ka strain and NIA
strain, which revealed identities higher than 99%. Northern
blot hybridization study revealed that the transcripts of gH is
2.3 kb in size (analog to Ka strain). The RNA is transcribed
from 2 to 8 hours after infection.
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