Comparison of the migration pattern of avian reovirus genome and characterization of the nucleotide sequence of virus strain S1133 S3 fragment

• Main Authors: Wu, Wan Yu, 吳婉瑜
• Other Authors: Lee, Long Huw
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/32364940455550761467

碩士 === 國立中興大學 === 獸醫學系 === 82 === RNA isolate from ARV strain S1133 was adenylated wite poly(A) polymerase.The poly(A)‧RNA was isolated by oligo(dT)-cellulose chromatography and was reversed transcribed with M-MLV reverse transcriptase. The resulting DNA was tailed with oligi(dC) with terminal transferase and was cloned into oligo(dG)-tailed pBR322 at the PstI site. W1 which contained 1-kb cDNA insert was sequenced.Result show that this fragment contained an open reading frame of 852 nucleotides,encoding a protein of 35.983 kDa.Dot-blot hybrydization results showed that the cDNA fragment was located on the S3 segment of ARV.The amino acid seqence of the putative protein was highly similar to the seqence of the outer capsid protein,σ3,encoded by the S4 segment of mamalian reovirus (MRV) .Therefore,the ARV open reading frame may also encode a protein of similar function. The results obtained from this study wil be useful for the understanding of ARV and for the diagnosis of the disease caused by the pathogen.