| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 82 === RNA isolate from ARV strain S1133 was adenylated wite poly(A)
polymerase.The poly(A)‧RNA was isolated by oligo(dT)-cellulose
chromatography and was reversed transcribed with M-MLV reverse
transcriptase. The resulting DNA was tailed with oligi(dC)
with terminal transferase and was cloned into oligo(dG)-tailed
pBR322 at the PstI site. W1 which contained 1-kb cDNA insert
was sequenced.Result show that this fragment contained an open
reading frame of 852 nucleotides,encoding a protein of 35.983
kDa.Dot-blot hybrydization results showed that the cDNA
fragment was located on the S3 segment of ARV.The amino acid
seqence of the putative protein was highly similar to the
seqence of the outer capsid protein,σ3,encoded by the S4
segment of mamalian reovirus (MRV) .Therefore,the ARV open
reading frame may also encode a protein of similar function.
The results obtained from this study wil be useful for the
understanding of ARV and for the diagnosis of the disease
caused by the pathogen.
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