| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 82 === The immunomodulatory potential of sodium diethyl-
dithiocarbamate (DTC), Ganoderma extract and interleukin-2
(IL-2) was assessed in vivo and in vitro study. For in vitro
study, the tetrazolium salt colorimetric assay (MTT) was
performed to evaluated cell proliferation. The result showed
that DTC and Ganoderma extract did not enhance the
proliferation induced by concanavalin A (Con A, 2 ug/ml). In
fact, the DTC (0.98-7.8 ng/ml) was toxic (>80% cell dead) to
splenocytes and yac-1 cells in culture. The presence of
Ganoderma extract did not demonstrate the inhibitory effect on
splenocytes and yac-1 cells. For in vivo study, mice were
treated with DTC (50 mg/kg b.w., i.p.) once and splenocytes
were prepared on 7 or 14-day after treatment. The splenocytes
of DTC-treated animals were showed an increase in mitogen-
induced proliferation in vitro. The values of %proliferation
were 100% for control group, 302% for phytohemagglutinin
(5%)-7-day group, 116% for lipopolysaccharide (5 ug/ml)-7-day
group, 182%for PHA-14-day group and 175% for LPS-14-day group.
Treatment with Ganoderma extract (3 mg glucose content/mouse ,
i.p.) did not enhance the splenocyte proliferation induced by
mitogen (Con A, PHA and LPS). However, mice fed with feed
containing Ganoderma powder (1.6 g/kg b.w./day) for 22 wk were
showed an enhancement of Con A (2 ug/ml)-induced proliferation
(158%). Five-day IL-2 treatment (6-9x103 units/mouse, i.p.)
resulted in enhancement of PHA response (369%) and LPS response
(478%). NK activity of mice treated with DTC, Ganoderma and
IL-2 (i.p.) was measured with LDH release assay. Because of
the high spontaneous release of effector cells (splenocytes),
the result was not suitable to judge the effect of DTC,
Ganoderma on NK activity. However, these substances did
slightly increase the NK activity of treatment group. In
addition, the phagocytic activity assessed with NBT stain was
enhanced by the treatment with DTC, Ganoderma and IL-2 .
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