| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 82 === The objective of this study is to prepare monoclonal
antibodies (MAbs) against the hemolysin of Actinobacillus
pleuropneumoniae (A.P.) serotype 1 (HylI). These MAbs were
then used in the injection of mice, to assess their
protection activity against A.P. challenge. The spleens of
BALB/c mice had been immunized 4 times with purified HylI
and showed very strong antibody response.These spleen cells
were then fused with P3x myeloma cells. When screening test
for specific antibodies production using the ELISA test, 24
out of 880 wells showed strong positive reactions. Subsequently
3 consecutive subclonings had established 9 strains of
hybridoma with stable activities of MAbs secretion. Two
strains among them with resist-hemolytic reactions
injected mice. When comparing those immunized mice with
those have been immunized by the bacteria, the survival
ratio was 8:5 after the A.P. challenge. Whereas the
contral groups (immunized with PBS) were killed after the
challenge. Those challenge bacteria could be recovered from
the lungs of dead mice.
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