The effects of sucrose、 PPF and CO2 enrichment on the growth of rose shoot explants.

• Main Authors: Anna Wu, 吳安娜
• Other Authors: Chein-Young Chu
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/92673024739416146791

碩士 === 國立中興大學 === 園藝學系 === 82 === Effects of sucrose concentration,photosynthetic photon flux( PPF) and CO2 enrichment on the growth of shoot explants of ''Samantha'',''Landora'' and ''Pitica'' roses were studiedin vitro. The level of sucrose in medium was at 3.8、7.5、15 or 30g/L.The more growth of ''Samantha'' and ''Landora'' explants was cultured on the medium contained higher concentration of sucrose. However, the best growth of ''Pitica'' explants was cultures on the medium contained 15g/L sucrose. And the multiplication rate of shoot explants increased as the sucrose concentration increased in medium.The CO2 concentration in vessel was higher when the sucrose concentration of culturing medium was higher. The growth of ''Samantha''and ''Landora''explants cultured under high PPF(70.mu./m2/sec) was greater than that cultured under low PPF (35.mu.mol/m2/sec).Moreover,under high PPF,the growth of explants cultured on a medium contained 15g/L sucrose was greater than that cultured on a medium contained 30g/L sucrose. Under high PPF,''Landora'' explants did not survive on a medium without sucrose but 40% of ''Samantha''explants survived.After cultured under high PPF for 4 weeks, the lowest CO2 concentration in vessel was in which the explants grow on a medium contained 15g/L sucrose. The dry matter of explants was higher and the leaves was dark green with CO2 enrichment. Explants cultured on a medium contained 7.5g/L sucrose and enriched with CO2 was the vigorest and a medium contained 15g/L sucrose enriched with CO2, the leaves of ''Samantha'' explants was distortion and the low leaves of ''Landora'' explants was yellowing.Under high PPF, on the leaf of explants there were ellipsoid stomata with different aperture. Furthermore, the leaf had typical palisade mesophyll and there wrer starch grains in chloroplast. The additional CO2 enrichment culture, the aperture of stomata was smaller or closed, and the leaf thickness was greater and there was the biggest starch grain if the explants cultured on a medium contained 7.5 g/L.