Studies of The Vasodilating Effect of Berberine in Rat Denuded Aorta
碩士 === 高雄醫學大學 === 醫學研究所 === 82 === Berberine, an alkaloid isolated from the Chinese herb Huanglian (the rhizome of Coptis Chinensis Franch) has been reported to have a vasodilating effect in rats and dogs. The vasodilating effect of berberine was assessed by adding phenylephrine (PE) or hi...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
1994
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/28437504607721097696 |
| Summary: | 碩士 === 高雄醫學大學 === 醫學研究所 === 82 === Berberine, an alkaloid isolated from the Chinese herb Huanglian (the rhizome of Coptis Chinensis Franch) has been reported to have a vasodilating effect in rats and dogs. The vasodilating effect of berberine was assessed by adding phenylephrine (PE) or high potassium in the presence of various concentration of berberine. The effect of berberine on PE-sensitive Ca store was evaluated by adding PE to aorta in Ca-free solution. A7r5 smooth muscle cells with whole-cell patch clamp technique were used to study the effect of berberine on Ica, L of smooth muscle cells.
The results show that berberine (0.5-50 uM) inhibited aorta contraction induced by PE, but did not decrease the high potassium induced contraction. Berberine (30 uM) did not change the Ica, L of A7r5 cultured smooth muscle cells.
Conclusion :
1. The vasodilating effect of berberine is independent of the lining endothelium.
2. Berberine does not have significant inhibition on voltage dependent L-type calcium channel in A7r5 smooth muscle cells. And Nifedipine significantly inhibits L-type calcium channel.
3. The vasodilating effect of berberine appears to be related to the inhibition of phenylephrine-induced calcium release from sarcoplasmic reticulum.
4. To observe the effect of berberine on the potassium current of smooth muscle cells is helpful to understand the mechanism of vasodilating effect of berberine.
|
|---|