| Summary: | 碩士 === 輔仁大學 === 食品營養學系 === 82 === The purpose of this study was to evaluate the effect of dietary
protein sources and levels on immune efficacy of Balb/c mice
with hepatitis B vaccination. Mice fed 4, 12, 20% and 30%
casein or soyprotein diet were immunized with hepatitis B
vaccine after 7 days adapted periods. During the experimental
period, it was found that the net food intake, splenocyte
number/spleen weight, activity of serum glutamate oxaloacetate
transaminase (GOT), glutamate pyruvate transaminase (GPT),
lactate dehydrogenase(LDH) were not significantly different
between the 4 protein level groups. However, body weight,
spleen weight, total splenocyte number, serum total protein and
albumin concentration were found to be lower in 4% protein
containing group as compared with the 12,20 and 30% protein
groups. There is no significant differences on anti-HBsAb titer
in mice with hepatitis B vaccination between casein and
soyprotein group. However, mice fed 4% protein exhibited a
higher tendency in getting higher anti-HBsAb titer and also T-
cell, T-helper cell (Th), T suppressor/cytotoxic cell (Ts)
percentage. Besides, an increased PHA stimulated splenocyte
proliferation was found in 4% protein group. B cell percentage
was reduced in 4% protein group as compared with the 12, 20 and
30% protein groups. PWM stimulated splenocyte proliferation was
not affected by dietary protein sources and levels. Therefore,
low protein diet would raise the anti-HBsAb titer in hepatitis
B vaccinated mice by modifying the immune system: T-cell, Th,
Ts and B cell percentage.
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