The Study of Antiviral Effect on Folk Medicines (2) The Study of The Quality Control on Chinese Medicines (4)

• Main Authors: Mei-Hsien Lee, 李美賢
• Other Authors: Prof. Kun-Ying Yen
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/51090718377718824780

碩士 === 台北醫學院 === 藥學研究所 === 81 === Recently, the polyphenol compounds containeds in natural plants which were researched in antivirus to consider important. Taiwan is located in the subtropical zone and there are aboundant plants in Taiwan.Using chemical methods to screen Taiwan folk medicines which contained polyphenols and anti-EBV (Epstein-Barr virus) DNA polymerase activity in vitro which is associated with nasopharyngeal carcinoma (NPC). Eugenia uniflora Linn. is one of the active folk medicines. By applied of various chromatographic and spectroscopic techniques to the isolation, analysis and structure determination of these compounds, such as: column chromatography、high-performance liquid chromatography (HPLC)、 FAB-MS、UV spectra、IR spectra、1 H-NMR spectra、13C-NMR spectra、 CD spectra and so on. There were four crystal compounds, which were isolated from ethyl acetate fraction of the Eugenia uniflora, gallocatechin and myricitrin, are testing for anti-EBV DNA poly- merase activity. In addition the mechanism of activity was used the cell lines (eg: Raji cells) to do further study. Another research is using HPLC method to quantity of gentio- picroside which is the conducive component to the health of stomach. The best yield of the Long-Dan in different extraction conditon was investigated. In addition, in various drug markets and the various contents of gentiopicroside in the Chinese medicinal prescriptions containing Long-Dan were also performed. The results were found 100 volume of Long-Dan(W/V) of 50﹪ methanol in 50 minutes of sonication could produce the best yield. The contents of gentiopicroside in various drug markets were found in range from 1.30﹪ to 5.86﹪.