Methylation Analysis and Immuno-Flow-cytometric Studies on an Antitumor Polysaccharide PN-2 Puritied from Phytocordyceps nin- chukispora Su et Wang

• Main Authors: Tsun-Cheng Kuo, 郭俊成
• Other Authors: Ching-Hua Su 、 I-CHI TUNG
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/03677498638010057104

碩士 === 台北醫學院 === 天然物醫學研究所 === 81 === Phytocordyceps ninchukispora was a new species of the new genus Phytocordyceps in the family of clavicipitaceae found in Taiwan in 1985. In the liquid culture of P.ninchukispora, a water soluble polysaccharide (PN-2) was isolated. This polysaccharide was used for chemical structure and antitumer studies.Based on the data obtained from chitinase assay and GC,GC/mass spectrome- try, PN-2 appeared to be a polymer of β-1-4 N- acetylglucosamine as the main chain with a molecular weigut approxinately 1*10 ． The side chains of galactose, mannose and threitol meioties were linked by β 1-4-linkage to the 6 position of glucosamine residues of the polymer. PN-2 reduced subcutaneous tumor size by 29% and prolonged the life span of mice implanted with S-180 cells by 21%. PN-2 also reduced the nodule number in munine lung by 88% as demonstrated by artificial metastasis test of the 14th day after S-180 cell implantation. A flow-cytometric mesaurement was established to distinguish normal lung cell and S-180 cell in the present study. PN-2 had no direct cytotoxic effect on tumor cells and was neutral in the Ames test. The antitumor activities were evidenced by increasing total number of T cells, T4 cells and macrophages as demonstrated by flow cytometric analysis. PN-2 dramatically reduced the viability of the tumor cell as indicated by BrdVrd and PI staining,but had no effect on labeling index of Brdulrd, DNA synthesis time (Ts) and G1/S/G2M ratio. I.p. administration of PN-2 strongly activated macrophages and a prominent mitogenic effect which resulted in increasing WBC division ability. I.V.administration of PN-2 in mice did not change the peripheral blood profiler inclluding the percentage of WBC.RBC. PLT.HGB%L,%M,RDV,PDW,LYM,GRAN,HCT, MCH and PCT;but slightly increased the values of MCV and MCHC.