| Summary: | 碩士 === 台北醫學院 === 天然物醫學研究所 === 81 === Phytocordyceps ninchukispora was a new species of the new genus
Phytocordyceps in the family of clavicipitaceae found in Taiwan
in 1985. In the liquid culture of P.ninchukispora, a water
soluble polysaccharide (PN-2) was isolated. This polysaccharide
was used for chemical structure and antitumer studies.Based on
the data obtained from chitinase assay and GC,GC/mass
spectrome- try, PN-2 appeared to be a polymer of β-1-4 N-
acetylglucosamine as the main chain with a molecular weigut
approxinately 1*10 ． The side chains of galactose, mannose and
threitol meioties were linked by β 1-4-linkage to the 6
position of glucosamine residues of the polymer. PN-2 reduced
subcutaneous tumor size by 29% and prolonged the life span of
mice implanted with S-180 cells by 21%. PN-2 also reduced the
nodule number in munine lung by 88% as demonstrated by
artificial metastasis test of the 14th day after S-180 cell
implantation. A flow-cytometric mesaurement was established to
distinguish normal lung cell and S-180 cell in the present
study. PN-2 had no direct cytotoxic effect on tumor cells and
was neutral in the Ames test. The antitumor activities were
evidenced by increasing total number of T cells, T4 cells and
macrophages as demonstrated by flow cytometric analysis. PN-2
dramatically reduced the viability of the tumor cell as
indicated by BrdVrd and PI staining,but had no effect on
labeling index of Brdulrd, DNA synthesis time (Ts) and G1/S/G2M
ratio. I.p. administration of PN-2 strongly activated
macrophages and a prominent mitogenic effect which resulted in
increasing WBC division ability. I.V.administration of PN-2 in
mice did not change the peripheral blood profiler inclluding
the percentage of WBC.RBC. PLT.HGB%L,%M,RDV,PDW,LYM,GRAN,HCT,
MCH and PCT;but slightly increased the values of MCV and MCHC.
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