Bosynthesis of Vitamin C By Fermentation --L-sorbose Pathway

• Main Authors: Lim, Yeik Hwa, 林月華
• Other Authors: Yang, Fan Chiang
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/88553117571070718540

碩士 === 東海大學 === 化學工程研究所 === 81 === In this reseach D-sorbitol was biotransformed into L-sorbose by Acetobacter pasteurianus for the biosynthesis of the precursor of Vitamin C. The experimental items included(1) The effects of aeration, innoculum, initial substrate coccentration and initial pH value on the production of L-sorbose in batch culture. (2)Im- mobilization of living cells in different calcium alginate con- centration gels. (3) The investigation of continuous operation using an immobilized packed-bed bioreactor. Based on substrate inhibition effects, a kinetic model for both bacterial growth and product formation was proposed. D-sorbitol concentration more than 8% showed inhibition effects on both cell growth and L-sorbose production. The immobolized bacterial systems in both entrapment and adsorption were proved to be more efficient than suspension culture.