Factors on the Biodegration of Aliphatic Hydrocarbon

• Main Authors: Wu,Khwa Chan, 吳國權
• Other Authors: Chao,Wei Liang
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/90595646057158142249

碩士 === 東吳大學 === 微生物學系 === 81 === Pollutants of petroleum origin consisted mainly of aliphatic hydrocarbons. Alkane biodegradation made up of the major portion of work dealing with treating petroleum pollutants. In this stu- dy, ten bacterial isolates and one yeast isolate were obtained from the oil polluted terrestrial and marine environments using hexadecane as a sole carbon source. Besides hexadecane, these isolates could also use a wide range of other alkanes for growth. Nine of these ten bacterial isolates belong to the genus Acine- tobacter. By using Biolog system, they were identified as Acine- tobacter haemolyticus, A. baummnnii and A. calcoaceticus. In ge- neral, bacteria degrade hexadecane faster than yeast. The rates of degradation of hexadecane by Acinetobacter spp. were the highest around neutral pH. In yeast, the rate of degradation was decreased above pH8.0 but was not affected at pH 3.5. When hexa- decane was used as the sole carbon source, the increase of bio- mass (i.e.,protein content) for both bacteria and yeast were the slowest at 15℃.Although lower incubation temperature will lower the rate of biomass accumulation but eventually the amount of protein in the system were the same regardless whether it was incubated at 15℃, 25℃ or 30℃. However, different results were observed for the yeast which produced the largest amount of bio- mass while incubated under 30℃ followed by 25℃ and then 15℃. When incubated in the presence of 3%NaCl and 0.1%KCl, an extent- ed lag phase was detected for A. haemolyticus isolate SCU4 but not in the case of yeast. In media with C/N around 50/1, hexade-