| Summary: | 碩士 === 東吳大學 === 微生物學系 === 81 === Pollutants of petroleum origin consisted mainly of aliphatic
hydrocarbons. Alkane biodegradation made up of the major
portion of work dealing with treating petroleum pollutants. In
this stu- dy, ten bacterial isolates and one yeast isolate
were obtained from the oil polluted terrestrial and marine
environments using hexadecane as a sole carbon source.
Besides hexadecane, these isolates could also use a wide
range of other alkanes for growth. Nine of these ten bacterial
isolates belong to the genus Acine- tobacter. By using Biolog
system, they were identified as Acine- tobacter haemolyticus,
A. baummnnii and A. calcoaceticus. In ge- neral, bacteria
degrade hexadecane faster than yeast. The rates of degradation
of hexadecane by Acinetobacter spp. were the highest
around neutral pH. In yeast, the rate of degradation was
decreased above pH8.0 but was not affected at pH 3.5. When
hexa- decane was used as the sole carbon source, the increase
of bio- mass (i.e.,protein content) for both bacteria and
yeast were the slowest at 15℃.Although lower incubation
temperature will lower the rate of biomass accumulation but
eventually the amount of protein in the system were the
same regardless whether it was incubated at 15℃, 25℃ or
30℃. However, different results were observed for the yeast
which produced the largest amount of bio- mass while incubated
under 30℃ followed by 25℃ and then 15℃. When incubated in
the presence of 3%NaCl and 0.1%KCl, an extent- ed lag phase was
detected for A. haemolyticus isolate SCU4 but not in the case
of yeast. In media with C/N around 50/1, hexade-
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