Beta-1,3-D-glucan from broth of Ganoderma tsugae that activates the human complement system and using coated C1q piezoelectric quartz biosensor to screen materials that activates the human

• Main Authors: Lee, Min-Yen, 李明彥
• Other Authors: Wang,Hsi-Hua;Tsai,Hsin;Lii,Cheng-yi
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/04639513642391226643

博士 === 國立臺灣大學 === 農業化學系 === 81 === This thesis containes two parts. Part 1 is concerned with the structural, conformational and biological analyses of polysacch- arides from the broth of Ganoderma tsugae. Part 2 is concerned with the application of piezoelectric quartz crystal for develop- ment of biosensor in liquid phase. Part 1. The extracellular polysaccharides were prepared by the precipitation from the broth of Ganoderma tsugae with ethanol, after 14 days of shaking culture. The yield of polysaccharides was about 2.5g/L. Only glucose was found in the acid hydrolyzate of the polysaccharide by HPLC. The polysaccharide was submitted to methylation, GLC-MS and 13C NMR analyses and was identified as a β-1,3-D-glucan having one branch length was one glucosyl unit. Gel chromatography with Fractogel HW-65( S) was applied for the fractionation of the β-1,6;1,3-glucan. One fraction (mw 219,500) was collected when eluented with 0.5% NaCl, and two fractions (mw 219,500 and 72,500) were collected with 0.5% NaOH. The thermal conformation was examined by DSC. One endothermic peak was observed at 131℃, which was assigned to the conformational tran- sition of the triple-helix into single chain. That thermal dena- turation of native triple- helix into the single chains was comp- lete in 10min at 150℃, had been proved by gel chromatography. These results showed that the polysaccharide isolated from G. tsugae is similar to the structur and conformation of schizophy- llan, an antitumor polysaccharide. Schizophyllan which has marked antitumor activity in hosts appears to represent host defense potentiators. The alternative complement pathway was assumed to be important in the first step of host defense mechanisms. The activation of human alternative complement pathway by β-1,6;1,3- D-glucan of G. tsugae was proved by haemolytic analysis.