| Summary: | 碩士 === 國立臺灣大學 === 園藝學系 === 81 === That shoot apex about 3 mm in length with partial cotyledon was
cultured on MS medium containing 2 mg/l BA stimulating lateral
bud proliferation. Adding GA 0.1 mg/l could enhance shoot
proliferation, Fifty to eighty budlets were proliferated after
4 to 5 times subculture on MS medium contained GA 0.1mg/l and
BA l. Sucrose was beneficial for cutting grouth and root ,
obtained 96% rooting while 60 g/l sucrose was added in absence
of auxinImmature embryos about 2-4 mm long cultured on medium
contained BA 1 mg/l, Kinetin 0.2 mg/l, NAA 0.5 mg/l and GA 0.1
mg/l, BA 2 mg/l can directly induce adventitious bud formation.
On MS medium containing BA 2mg/l and 2,4-D 0.2 mg/l, 5-day-old
sedling's cotyledon dedifferentated into compact callus, than,
it transfered to medium contained BA 0.2mg/l and 2,4-D 0.1mg/l
could regenerate plantlet on low frequency. leaf of multiple
shoot culture in 2,4-D 、NAA 0.2 mg/l medium was also inducible
to form a lot of budlets, and plantlets on containing NAA 0.6
mg/l, IBA 0.3 mg/l and Kinetin 0.2mg/l medium established. The
10∼20% immature zygote embryos of five cultivans were
indulible to regenerate Somatic embryos. Among basel medium MS.
SH. B5 and NN, only showed that the MS formulation was suitable
for somatic embryo- genesis. In comparation of Cytokinin BA ,
2ip, Kinetin and Zeatin. the former There exhibited more
efficiency than zeatin. The types of auxin also effected the
somatic embryogenesis. NAA showed strength than 2,4-D. and both
IAA and IBA were without effect on induction of somatic
embryogenesis. on MS medium contaning BA 2 mg/l and 2,4-D、NAA
0.2 mg/l, using plantlet's leaves derived from multiple shoot
as explant can induced abnornal Somatic embryos lacking shoot
primordia. On BA 2 mg/l and NAA、2,4-D 0.2 mg/l medium, using
abnormal leaves derived from multiple shoot as explant can
induce abnormal somatic embryos lacking shoot primordia.
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