| Summary: | 碩士 === 國立臺灣大學 === 園藝學系 === 81 === In the present studies, embryogenic callus of Panax ginseng C.A.
Meyer and leaves of Drimiopsis kirkii Baker were used for gene
transformation with a reporter gene (β-glucuronidase gene ,GUS
gene) by using particle bombardment techniques. Bombardment
explants were then cultured on MS (Murashige & Skoog) medium
for embryoid induction. The regenerated embryoids, plantlets
and the adjacent callus tissue were then examined to
demonstrate the exi- tence of exogenous GUS gene. Under control
of the CaMV 35S promotor, the GUS gene con- tained pBI221 (3
kb) were coated to gold particles (diameter 1.6μｍ) and were
delivered to targey explants by a setup (PDS1000/ He) od Bio-
Rad.Optimal bombardment conditions were found around 650 psi
(pounds square inch), 1/4-inch Gap, 1 spacer ring (8 mm) ,
25-inch Hg vacuum, 6-cm sample distance and 2 shots. Bombarded
explants of P. ginseng C.A.Meyer and D. kirkii Baker expressed
the foreign gene activity as checked with X-gluc assays two day
the bombardment. Stably expression of GUS gene was found in
leaf explants of D. kirkii Baker. However, only temporary
expression of GUS gene activity occurred in the bombarded
explants of Panax ginseng C.A.Meyer. The pBI121 (13 kb)
plasmids were also used for gene trans- formation. Leaves of D.
kirkii Baker were transformed with pBI 121 plasmids
constructing the GUS and NPT II genes. The bombard- ment
coditions were as same as the pBI221 treatment. Transformed
explants were cultured on MS medium containing 150 mg/l Kanamy-
cin and then produced white embryoids.
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