| Summary: | 碩士 === 國立臺灣大學 === 動物學系 === 81 === Human pituitary gland tumor cells (Hpt-1) were transfected with
a maternal gene from carp egg. A transfectant cell line Hp-3
lost the ability to growth in soft agar. Immunoprecipitation
and western blot analysis revealed that in Hp-3 the protein
amount of epidermal growth factor receptor (EGFR) was
considerably reduced as compared with the parental Hpt-1 cells,
on the other hand that of glial fibrillary acidic protein
(GFAP) and p53 were increased. Furthermore we found that Hp-3
cells could adhere and proliferate in bacteriological dish
better than Hpt-1 cells, which suggested that Hp-3 cells
secret a lot more of extracellular matrix into the medium. The
result of western blot analysis indeed showed that the protein
level of fibronectin of Hp-3 cell grown in bacteriological
dishes was increased as compared with that in tissue culture
dishes. A growth-stimulating activity was found in the serum-
free medium conditioned by Hpt-1 or Hp-3 cells, which promoted
the proliferation of hepatoma (NTU-BL), Hela, NIH 3T3 and human
fibroblast cells except Hpt-1 and Hp-3 cells themselves. And
there was no significant difference in the growth-stimulating
activity between Hpt-1 and Hp-3 conditioned media. Hela and
3T3 were chosen as the main assay systems and exhibited a dose-
dependence of mitotic response to the added conditioned medium.
Since basic fibroblast growth factor (bFGF) has been examined
as an autocrine regulator of glioma cell growth and a paracrine
angiogenic factor facilitating its neoplastic progression, 0.5
ug /ml of anti-human bFGF antibody was added to the conditioned
media of Hpt-1 and Hp-3 cells. However, the growth-stimulating
activity was still not neutralized, suggesting that the
mitogenic activity was not from bFGF.
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