The Studies of Growth and Differentiation Properties of Carp Maternal Gene Transfected Tumor and Embryonic Cells

• Main Authors: Yeh,Pei-Yen, 葉培彥
• Other Authors: Yew, Fu-Shie
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/55471666542199085330

博士 === 國立臺灣大學 === 動物學系 === 81 === A carp maternal cDNA library was transfected into tilapia ovary cells, and on the basis ofcell clonalgrowth rate, a single clone which showed slower growth rate was selected, and a plasmid, de- signated pSC19, was rescued from that clone. Transfection of pSC19 into a human hepatoma cell line showed the transformed cells lost their tumor cell properties, unable to pile -up andgrow in soft agar. In thetransfectant, oncogenic proteins includingras, src, abl, EGF receptor as well as a hepatoma marker -fetoprotein were decreased in transfectant. However the expression oftumor suppre- ssor p53 was enhanced. Furthermore, a tumor growths pecific inhi- bitor was characterized in medium conditioned by thetransformed cells. Similar characteristics were observed in pSC19 transfected HeLa cells. The transfectants lost the abilities to grow in soft agar and to form tumor in nude mice,in accompany with the increa- sinp 53 protein.This maternal gene also showed the ability to in- duce differentiation following transfection into NIH3T3 cells. pSC19 transfected 3T3 cells appeared morphologically as neuron- glial cells, and biochemical differentiation markers including neuronalfilament, glial fibrillary acidic protein,keratin and tro- poninT were observed by cytoimmunostain and western blot analysis From the results ofexperiments performed in cultured cells, this maternal gene was expected to be involved in cell growth and dif- ferentiation control.