| Summary: | 博士 === 國立臺灣大學 === 動物學系 === 81 === A carp maternal cDNA library was transfected into tilapia ovary
cells, and on the basis ofcell clonalgrowth rate, a single
clone which showed slower growth rate was selected, and a
plasmid, de- signated pSC19, was rescued from that clone.
Transfection of pSC19 into a human hepatoma cell line showed
the transformed cells lost their tumor cell properties,
unable to pile -up andgrow in soft agar. In thetransfectant,
oncogenic proteins includingras, src, abl, EGF receptor as well
as a hepatoma marker -fetoprotein were decreased in
transfectant. However the expression oftumor suppre- ssor p53
was enhanced. Furthermore, a tumor growths pecific inhi- bitor
was characterized in medium conditioned by thetransformed
cells. Similar characteristics were observed in pSC19
transfected HeLa cells. The transfectants lost the abilities
to grow in soft agar and to form tumor in nude mice,in
accompany with the increa- sinp 53 protein.This maternal gene
also showed the ability to in- duce differentiation following
transfection into NIH3T3 cells. pSC19 transfected 3T3 cells
appeared morphologically as neuron- glial cells, and
biochemical differentiation markers including neuronalfilament,
glial fibrillary acidic protein,keratin and tro- poninT were
observed by cytoimmunostain and western blot analysis From the
results ofexperiments performed in cultured cells, this
maternal gene was expected to be involved in cell growth and
dif- ferentiation control.
|
|---|
