| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 81 === Bilirubin from pig bile was analyzed in this experiment. The
bilirubin diazo products, obtained from diazo reaction using
diazotized o-ethyl anthranilate as the diazo reagent, were
analy- zed by UV-VIS and high performance liquid
chromatography. After diazotization, the diazo products were
also separated with column chromatography and the main
fractions furthermore underwent tran- sesterifica-tion and
acetylation. NMR and Mass spectra of these reaction products
were used as the corroborative evidence for the previous
analysis. Results of these investigations showed that there
were probably three types of bilirubin existed in pig bile
,namely, alpha,beta,and gamma forms. The phenol-sulfuric
acid method was used to measure the total amount of sugar
released from bilirubin after transesterification. The
quantitation showed that one molecule of bilirubin, on the
average, was combined with about 1.49 molecule of glycosyl
group. It was also found that the amount of bilirubin in
pig bile was different among pigs; about 12 to 23
miligram/100 mililitre.
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