Foam Fractionation of Proteins

• Main Authors: Wang,Sheng-Shih, 王勝仕
• Other Authors: Liu,Hwai-Shen
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/30186609347316538693

碩士 === 國立臺灣大學 === 化學工程研究所 === 81 === A batch-type and a continuous-type foam fractionation column to separate and purify Bovine serum albumin (BSA)protein solution was studied experimentally. The effects of several important operating variables such as gas flow rate, temperature, protein concentration and pH value were investigated. Besides, the foam- sampling height, foam-column diameter and salt additives were also studied. The separation ratios (SR) were found to increase with decreasing protein concentration in the experimental range, and the curve of separation ratio vs pH values yields an optimal curve with the maximal SR near pH 5.0, which is the isoelectric point of BSA. The experimental results also indicated that SR increases as decreasing gas flow velocity and the addition of , while temperature shows little effect. Similarly, the same conclusions were also obtained in the continuous system. It is worthwhile mentioning that the dimensions of foam column did affect the separation performance; i.e. the higher sampling height and smaller foam column diameter can both enhance the separation ratio and foam concentration. Moreover, the quasi -equilibrium curve for the BSA-solution was also sought experimentally. Based on the knowledge of fluid mechanics, colloidal science and mass balance principle, a related mathematical model was developed. Though there exist several assumptions, qualitatively, the model was found to be in good agreement with the experimental data.