| Summary: | 碩士 === 國立臺灣大學 === 化學工程研究所 === 81 === A batch-type and a continuous-type foam fractionation column
to separate and purify Bovine serum albumin (BSA)protein
solution was studied experimentally. The effects of
several important operating variables such as gas flow rate,
temperature, protein concentration and pH value were
investigated. Besides, the foam- sampling height, foam-column
diameter and salt additives were also studied. The
separation ratios (SR) were found to increase with
decreasing protein concentration in the experimental range, and
the curve of separation ratio vs pH values yields an
optimal curve with the maximal SR near pH 5.0, which is the
isoelectric point of BSA. The experimental results also
indicated that SR increases as decreasing gas flow velocity
and the addition of , while temperature shows little
effect. Similarly, the same conclusions were also obtained in
the continuous system. It is worthwhile mentioning that the
dimensions of foam column did affect the separation
performance; i.e. the higher sampling height and smaller foam
column diameter can both enhance the separation ratio and
foam concentration. Moreover, the quasi -equilibrium
curve for the BSA-solution was also sought
experimentally. Based on the knowledge of fluid mechanics,
colloidal science and mass balance principle, a related
mathematical model was developed. Though there exist several
assumptions, qualitatively, the model was found to be in good
agreement with the experimental data.
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