Determination of the Bioactive Components in Ligustici Rhizoma

• Main Authors: Jing-Woei Mou, 牟經偉
• Other Authors: Shuenn-Jyi Sheu
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/15840975956803139402

碩士 === 國立師範大學 === 化學學系 === 81 === Ligustici Rhizoma is a widely used Chinese herbal drug which contains essential oils, organic acids, phenols, alkloids and carbohydrates as its main components. Pharmacological tests revealed that those essential oils and organic acids had the functions of inhibiting cramped, sedating, depress- ing the blood level and antifungaling. In this study we used nicotinic acid, tetramethylpyrazine, ferulic acid, butylphthalide, senkyunolide A , ligustilide and butylidenephthalide as the reference standards to evaluate the quality of commerical crude drugs by means of high- performance liquid chromatography ( HPLC ). The scientific significance of hebral processing were also investigated. The analytical data showed that the contents of the commerical crude drugs were as follows: nicotinic acid 0 - 0.05 mg/g, tetramethylpyrazine 0 -0.04 mg/g, ferulic acid 0.49 - 1.10 mg/g, butyl- phthalide 0.14 - 0.62 mg/g, senkyunolide A 0.45 - 1.00 mg/g, ligustilide 4.00 - 8.55 mg/g, butylidene- phthalide 1.33 - 2.19 mg/g. Basically the contents of Ligustici Rhizoma were closely related to their apperance. According to the results, it was found that the contents of extractions were all increase after being processed by different processing methods. Those methods and their suitable processing times were as follows: fried until became yellow in color - 40 minutes, fried with wine - 10 minutes, soaked with water - 3 hours, steamed with wine - 30 minutes . Among the above processing methods, that of fried with wine could give excellent result. The organic acids such as nicotinic acid , ferulic acid, p-hydroxybenzoic acid, caffeic acid, protocatechuic acid, vanillic acid etc. had also been subjected to capillary electrophoresis ( CE ) and a satisfactory separation was obtained too. Nevertheless a further insight investigation was still needed.