Somatic Embryogenesis from Immature Embryo Culture of Peanut (Arachis hypogaea L.)

• Main Authors: Lai Yan-Min, 賴媛敏
• Other Authors: Yeh Mau-Shing
• Format: Others
• Language: zh-TW
• Published: 1993
• Online Access: http://ndltd.ncl.edu.tw/handle/04252171666282418420

碩士 === 國立中興大學 === 農藝學系 === 81 === The objective of this study was to investigate the effect of medium composition on somatic embryogenesis from immature embryo of different pod development stages,embryo ages and seed lengths in peanut. Two cultivars, i.e., Tainan selected 9 and Tainan 11, and six media were used in the experiment. Took L2 medium as the basis, three media, denoted by A1, A2 and A3, were prepared with the addition of(1) 2 mg/l NAA + 0.01 mg/l Kinetin + 2 mg/l ADE, (2) 2 mg/l NAA + 1 mg/l BA, and (3) 0.02 mg/l PIC + 40mg/l ADE + 0.05mg/l ABA, respectively. An A4 medium was prepered by adding 2.5 mg/l 2,4-D into the MSB basal medium. The rest two media, A5 and A6, wereprepered with MS medium as the basis and added by 1mg/l 2,4-D and 1mg/l 2,4-D + 1mg/l NAA,respectively.The results are summarized as follows: 1. Induction rate of somatic embryo changed with pod development stages. The somatic embryogenesis frequency of immature embryos from pods at R5 stage was higher than that from pods at R6 stage. 2. Among the six media used in this study, A1 medium was good for root formation, whereas the A2 medium induced many normal somatic embryos. The A3 medium gave many but abnormal somatic embryos. All the rest three media, A4, A5 and A6, induced a great amount of callus. 3. Immature embryos with an age under 11 days had low somatic embrypogenesis frequncy.However, one or two somatic embryos were observed beside the spread cotyledons or the original embryo axes derived from immature embryos with an age of 12-16 day. The immature axes of 17-20 day got rather high somatic embryogenesis frequency. 4. The immature seed shorter than 3 mm gave low somatic embryo- genesis frequency. However, the intact immature embryo cut from seeds with 3-6mm in length had high frequency of embryogenesis. As to the 6 mm or longer seed, their somatic embryogenesis rate depended on the media.