| Summary: | 碩士 === 國立中興大學 === 植物學系 === 81 === It was discovered that, a new isolated green alga
(Chlorophyceae) Chlorella sp. strain DT, could survive in
very desiccated condition. After drying 3 hours at 40 C , it
could restore growth 7 days later if rehydrating again in
liquid medium. The viability of this alga after desiccated
3 hours at 40C was investigated using three vital stains, Evans
Blue 、Fluorescein diacetate (FDA) and Nitro Blue Tetra- zolium
(NBT), and showed the rate was 97.65%、0.34% and 3.35%
respectively. It means that Chlorella sp. strain DT did
keep its membrane intact but lost its enzymes activity when
confronted very desiccated condition. And this may be one of
the mechanisms that makes it survive under desiccation. A
novel soluble protein of Chlorella sp. strain DT was
discovered when it was dry at 40 C after 2 hours. The
molecular weight of this novel protein was 250 KD. And it was
discovered at 40 C drying only, not at 60 C , 70 C or 40 C
water bathing. In addition, the protein bands of 60 KD and 70
KD increased after 2 hours drying at 40 C. Since 60 KD
and 70 KD proteis are similar to the known heat shock proteins
, and therefore , these proteins and novel 250 KD
soluble protein may play protecting role when this alga was
desiccated.
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