| Summary: | 碩士 === 國立中興大學 === 分子生物研究所 === 81 === Filamentous phages phiLf, phiXo and phiXv were isolated from
Xanthomonas campestris pv. campestris, X. oryzae pv. oryzae and
X. campestris pv. vesicartoria, respectively. To understand the
evolutionary relationships of Xanthomonas filamentous phages
and the mechanism of host-specificity, the adsorption protein
genes (gIII) of phages phiXo and phiXv were cloned and
sequenced for comparison. Gene III of phiXo was located in a
1.4 kb HindIII-KpnI fragment, phiXv gIII was in a 1.25 kb
HindIII-HincII fragment. Both gIII-containing fragments were
cloned and sequenced. Comparison of gIII sequences of phiLf,
phiXo and phiXv revealed several features. (1) They all used
GTG as start codon and contained weak promoter sequences which
were overlapped with the terminator pf gVIII.(2) The gIII
products contained conserved N-terminal signal peptide and C-
terminal anchor domains. (3) The constitution of glycine-rich
domains varied between phages.
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