| Summary: | 碩士 === 國立臺灣大學 === 農業化學研究所 === 65 === Our laboratory had studied the conjugation between ouxotrophs of glutamate producing bacteria in the past three years. Although no recombinant had been formed on selective medium, we had found the cross feeding of two ouxotrophs which closed contact that could grow on selective medium that makes us to consider the importance of cell contact Jensen (1952) proposed some phylogenetic rclation botween Corynebacterium and actinomycetes. Therefore, the properties of plasmids such as SCPI affecting recombination and catabolite repression of Streptomyees coelicolor A3 (2) were examined with the using of acridine orange. The transfer of gene or SCPI from S. coelicolor A3 (2) to gluamate producing bacteria by fusion between protoplasts of S. coelicolor A3 (2) and glutamate producing bacteria was also tried.
The results included:
The aerial mycelia formation of s. coelicolor A3 (2) strain 104 (IF) repressed by catabolite repression, but there was no catabolite repression in strain 1098 (UF). In strain 104 (IF), the number of spores and the SCPI loss frequency decreased as glucose concentration over 1.5% in complete medium. It means that the differentiation of aerial mycelia repressed partially as glucose concentration over 1.5% in complete medium. It seems like that this phenomenon favored the migration, distribution and replication of SCPI.
The maximum recombination frequency was 10-5 ~ 10-6 after rusion between protoplasts of auxotrophs of Brevibacterium divaricatum. There was no recombinant occurs after fusion between protoplasts of s. coelicolor A3 (2) and glutanate producing bacteria.
Electron micrographs showed that there was no pili on the surface of spore in s. coelicolor A3 (2). There was no influence of acridine crange to SCPI in (1) replication and migration in substrate and aerial mycelia (2) distribution, migration and replication in sporulation (3) replication of conjugal transfer. Acridine orange inhibited temporarily aerial mycelia formation of s. coelicolor A3 (2) on complete medium by inhibited transcription of some genes concerning aerial mycelia formation.
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