Chromatin Reassembly following a DNA Double-Strand Break Repair: The Ctf18-complex and Ctf4 work in concert with H3K56 Acetylation

• Main Author: Seepany, Harshika
• Other Authors: Greenblatt, Jack F.
• Language: en_ca
• Published: 2011
• Subjects: yeast; genetic interaction; DNA repair; acetylation; cohesion; histone
• Online Access: http://hdl.handle.net/1807/29616

The budding yeast, Saccharomyces cerevisiae, serves as an excellent model for identifying fundamental mechanisms of DNA repair. A Local Coherence Detection (LCD) algorithm that uses biclustering to assign genes to multiple functional sub-groups was applied on the chromosome E-MAP containing genetic interactions among genes involved in nuclear processes. Using this method, we found that Asf1 and Rtt109, genes that are together required for histone H3K56 acetylation, cluster together with Ctf4, Ctf18, Ctf8 and Dcc1, genes important for efficient sister chromatid cohesion. It is known that H3K56 acetylation is required for post-repair chromatin reassembly at sites of DNA double-strand breaks (DSBs). The cohesion genes were previously implicated in the repair of some DNA DSBs, but the nature of their involvement has not been reported. The experimental data in my thesis work suggest that Ctf4, Ctf8, Ctf18 and Dcc1 function in the post-repair chromatin reassembly pathway.