Dynamic Interleaved Imaging of Pyruvate Metabolism with Hyperpolarized 13C

• Main Author: Leung, Kevin Kai-Chi
• Other Authors: Cunningham, Charles H.
• Language: en_ca
• Published: 2010
• Subjects: 13C; hyperpolarized; metabolism; pyruvate; spectral-spatial; DNP; MR metabolic imaging; cancer; 0760; 0574; 0719; 0992; 0541
• Online Access: http://hdl.handle.net/1807/27311

Dynamic nuclear polarization and dissolution of 13C-labeled metabolite allows dynamic imaging of metabolism in-vivo. However, the spatial and temporal resolutions of magnetic resonance spectroscopic imaging are limited by the duration of free-induction decay acquisitions and the T1-based, non-recoverable polarization decay. This thesis describes the implementation of a spectral-spatial radiofrequency excitation pulse with a `flyback' echo-planar readout trajectory to dynamically image [1-13C]-pyruvate and [1-13C]-lactate in an interleaved manner. This technique excites a single resonance of either [1-13C]-pyruvate or [1-13C]-lactate and generates dynamic images with 5mm in-plane resolution. Metabolite dynamics extracted from the images and the corresponding non-localized spectroscopic data reveal similar kinetic rates upon fitting to a kinetic model. This demonstrates the feasibility of probing metabolism in heterogeneous tissues in-vivo with dynamic interleaved 13C MR imaging.