Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors
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Youngstown State University / OhioLINK
2015
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ndltd-OhioLink-oai-etd.ohiolink.edu-ysu14434753132021-08-03T06:33:29Z Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors Eisnaugle, Sarah L. Biology Genetics Statistics Glucan Cell wall Stress Penicillium marneffei Fungus Mutant Genetics Gene expression <i>Penicillium marneffei</i> is an opportunistic, dimorphic fungal pathogen that causes penicilliosis in AIDS patients. In an effort to genetically characterize this organism, Kummasook et al. developed an <i>Agrobacterium tumefaciens</i>-mediated transformation system in 2010. This system allowed for the production of thousands of <i>P. marneffei</i> mutant strains that could be further assessed in order to assess gene-to-function relationships. In 2014, Suwunnakorn et al., characterized one of these mutants, I231, and determined the presence of an interrupted <i>yakA</i> gene which codes for a dual-specificity tyrosine phosphorylation-regulated protein kinase. They determined that the <i>yakA</i> mutant produced fewer conidia, possessed increased chitin content, and exhibited unique cell-wall stress responses when compared to wild-type <i>P. marneffei</i>. The present study seeks to investigate these stress responses in the yakA mutant, a F4 wild-type strain, and the <i>yakA</i> complement strain CY21 at a genetic level. Using qRT-PCR, expression of three glucan synthase genes (<i>fksP, ags2</i>, and <i>kre6</i>) was quantified using the benA beta-tubulin gene as a reference gene for normalization. The three strains were subjected to cell-wall stressors including the anionic dye Congo red, the detergent sodium dodecyl sulfate, and the glucan synthase-specific antifungal drug caspofungun and assessed for glucan synthase gene expression. The resulting gene expressions indicated that the <i>yakA</i> mutant actually possessed relatively larger stress responses than the wild-type, indicating that the <i>yakA</i> gene and its resulting protein may be responsible for the regulation of glucan synthase gene expression in response to cell-wall stress. This reasoning is reinforced by the presence of increased chitin in the yakA mutant, a characteristic common to fungal mutants lacking proper glucan regulation in their cell-walls. 2015-09-29 English text Youngstown State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443475313 http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443475313 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology Genetics Statistics Glucan Cell wall Stress Penicillium marneffei Fungus Mutant Genetics Gene expression |
| spellingShingle |
Biology Genetics Statistics Glucan Cell wall Stress Penicillium marneffei Fungus Mutant Genetics Gene expression Eisnaugle, Sarah L. Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors |
| author |
Eisnaugle, Sarah L. |
| author_facet |
Eisnaugle, Sarah L. |
| author_sort |
Eisnaugle, Sarah L. |
| title |
Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors |
| title_short |
Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors |
| title_full |
Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors |
| title_fullStr |
Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors |
| title_full_unstemmed |
Glucan Synthase Gene Expression in<i> Penicillium marneffei</i> in Response to Cell-Wall Stressors |
| title_sort |
glucan synthase gene expression in<i> penicillium marneffei</i> in response to cell-wall stressors |
| publisher |
Youngstown State University / OhioLINK |
| publishDate |
2015 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443475313 |
| work_keys_str_mv |
AT eisnauglesarahl glucansynthasegeneexpressioninipenicilliummarneffeiiinresponsetocellwallstressors |
| _version_ |
1719439219370229760 |