Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation
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Youngstown State University / OhioLINK
2013
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ysu1377083669 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ysu13770836692021-08-03T06:19:24Z Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation Akpadock, Evelyn Biology Molecular Biology Microbiology Statistics Bioinformatics ufdB Penicillium marneffei ubiquitin dimorphism Agrobacterium tumefaciens-mediated transformation melanin <i>Penicillium marneffei</i> is a notorious, medically pertinent fungal pathogen responsible for causing penicilliosis within immunocompromised individuals, particularly those with HIV/AIDS. Existing as a mold at 25°C and yeast at 37°C, it is the only <i>Penicillium</i> species known to undergo a temperature-dependent dimorphic switch. Recently, <i>Agrobacterium tumefaciens</i>-mediated transformation (ATMT) was used to introduce randomly integrated T-DNA fragments into the <i>P. marneffei</i> genome. This study sought to genotypically and phenotypically characterize mutant I189. An inverse PCR protocol was employed to recover the DNA sequences flanking the T-DNA insertion site. BLAST analysis revealed an interruption of a putative ubiquitin fusion degradation protein (UfdB) mRNA. Orthologous in the human pathogen <i>Aspergillus fumigatus</i> as well as model yeasts <i>Schizosaccharomyces pombe</i> and <i>Saccharomyces cerevisiae</i>, this protein has been documented to tag abnormal stress-induced proteins for degradation. Current investigations of <i>ufdB</i> in <i>P. marneffei</i> mutant I189 show that fungal growth is significantly diminished in the presence of nutrient limitation although a full mRNA transcript is being produced. However, exposure to Congo Red, sodium dodecyl sulfate (SDS), and heat stress had no effect on cell viability. 2013-09-17 English text Youngstown State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ysu1377083669 http://rave.ohiolink.edu/etdc/view?acc_num=ysu1377083669 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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English |
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NDLTD |
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Biology Molecular Biology Microbiology Statistics Bioinformatics ufdB Penicillium marneffei ubiquitin dimorphism Agrobacterium tumefaciens-mediated transformation melanin |
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Biology Molecular Biology Microbiology Statistics Bioinformatics ufdB Penicillium marneffei ubiquitin dimorphism Agrobacterium tumefaciens-mediated transformation melanin Akpadock, Evelyn Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation |
| author |
Akpadock, Evelyn |
| author_facet |
Akpadock, Evelyn |
| author_sort |
Akpadock, Evelyn |
| title |
Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation |
| title_short |
Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation |
| title_full |
Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation |
| title_fullStr |
Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation |
| title_full_unstemmed |
Analysis of a <i>ufdB Penicillium marneffei</i> Mutant Generated by <i>Agrobacterium tumefaciens</i>-Mediated Transformation |
| title_sort |
analysis of a <i>ufdb penicillium marneffei</i> mutant generated by <i>agrobacterium tumefaciens</i>-mediated transformation |
| publisher |
Youngstown State University / OhioLINK |
| publishDate |
2013 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1377083669 |
| work_keys_str_mv |
AT akpadockevelyn analysisofaiufdbpenicilliummarneffeiimutantgeneratedbyiagrobacteriumtumefaciensimediatedtransformation |
| _version_ |
1719434753798569984 |