Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production

Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production

Bibliographic Details
Main Author: Bhadri, Shweta
Language:English
Published: Wright State University / OhioLINK 2021
Subjects:
Pharmacology
Cytokines
ultraviolet B radiation
UVB
intercellular communication
photosensitivity mechanisms
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=wright1622205404246803
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-wright16222054042468032021-08-03T07:17:36Z Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production Bhadri, Shweta Pharmacology Cytokines ultraviolet B radiation UVB intercellular communication photosensitivity mechanisms Cytokines play a pivotal role in regulating inflammation, which is a condition that makes the tissue vulnerable to different pathological and physiological conditions. Thus, how cytokines are regulated is an important area of study. Skin that receives ultraviolet B radiation (UVB), a major pro-oxidative stressor, results in the release of multiple cytokines and chemokines like tumor necrosis factor (TNF)-alpha and interleukin (IL)-8. Previous studies from our group and others have demonstrated synergistic release of TNF-alpha when UVB is combined with IL-1 or the lipid mediator Platelet-activating factor (PAF). Of interest, subcellular microvesicle particles (MVP) have been proposed to play an important role in intercellular communication. Moreover, UVB and PAF agonists cause MVP release in keratinocytes. Therefore, we believe that understanding the role of MVP in these inflammatory responses could be insightful for photosensitivity mechanisms and to suppress inflammation. The current study focuses on the combination of low concentrations of PAF agonist and UVB in-vitro and ex-vivo to observe potential synergism in the release of cytokines and MVP. We also studied the effects of acid sphingomyelinase (aSMase) inhibitor imipramine, for its ability to modulate both MVP and cytokine release. The application of aSMase inhibitor inhibited the synergistic response of MVP and cytokines allows us to conclude the potential involvement of MVP in the exaggerated response of cytokines from combining UVB and PAF. These studies have potential relevance in understanding abnormal skin reactions such as photosensitivity. 2021-06-04 English text Wright State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=wright1622205404246803 http://rave.ohiolink.edu/etdc/view?acc_num=wright1622205404246803 restricted--full text unavailable until 2022-06-04 This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Pharmacology
Cytokines
ultraviolet B radiation
UVB
intercellular communication
photosensitivity mechanisms
spellingShingle Pharmacology
Cytokines
ultraviolet B radiation
UVB
intercellular communication
photosensitivity mechanisms
Bhadri, Shweta
Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production
author Bhadri, Shweta
author_facet Bhadri, Shweta
author_sort Bhadri, Shweta
title Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production
title_short Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production
title_full Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production
title_fullStr Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production
title_full_unstemmed Potential Involvement of Micro vesicle Particles in the Synergistic Effects of Ultraviolet-B Radiation and Platelet -Activating Factor Receptor Agonists on Cytokine Production
title_sort potential involvement of micro vesicle particles in the synergistic effects of ultraviolet-b radiation and platelet -activating factor receptor agonists on cytokine production
publisher Wright State University / OhioLINK
publishDate 2021
url http://rave.ohiolink.edu/etdc/view?acc_num=wright1622205404246803
work_keys_str_mv AT bhadrishweta potentialinvolvementofmicrovesicleparticlesinthesynergisticeffectsofultravioletbradiationandplateletactivatingfactorreceptoragonistsoncytokineproduction
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