The role of substrate mechanics in nanotoxicity mediated by endocytosis
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Wright State University / OhioLINK
2017
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=wright1516127191655069 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-wright15161271916550692021-08-03T07:05:20Z The role of substrate mechanics in nanotoxicity mediated by endocytosis Boehm, Robert C. Toxicology PDMS Modulus toxicology A549 Presented in this work is the development of a novel variable modulus, porous PDMS-membrane based ALI cell culture model to assess cell growth under tissue matched stiffness conditions, tight-junction formation, and response to nanoparticle (NP) exposures. Using Sylgard 184 and 527 polydimethylsiloxane (PDMS) and corn syrup emulsions, produced 5 micron thick, highly porous membranes. Membranes spanning 1 kPa-1000 kPa have been achieved, modeling both healthy and fibrotic physiological lung tissue stiffness respectively. Scaffold microstructure and mechanics, cellular proliferation and viability under submerged and ALI conditions, were assessed. The viability was assessed using a Guava 12HT flow cytometer with Viacount reagent. Submerged exposure to 30 nm tannic acid stabilized gold or zinc oxide will be conducted for 8, 24, and 48 hours at 0, 1, 5, 10, 20, and 100 µg/ml. Inductively coupled plasma-mass spectrometry (ICP-MS) will be performed on the apical supernatants. A model system capable of capturing the unique mechanics and membrane properties found at the alveolar-endothelial interface could better predict toxicity, safe levels of exposure, and provide a rapid-means to assess new materials, pharmacologics, or potentially hazard chemicals. 2017 English text Wright State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=wright1516127191655069 http://rave.ohiolink.edu/etdc/view?acc_num=wright1516127191655069 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Toxicology PDMS Modulus toxicology A549 |
| spellingShingle |
Toxicology PDMS Modulus toxicology A549 Boehm, Robert C. The role of substrate mechanics in nanotoxicity mediated by endocytosis |
| author |
Boehm, Robert C. |
| author_facet |
Boehm, Robert C. |
| author_sort |
Boehm, Robert C. |
| title |
The role of substrate mechanics in nanotoxicity mediated by endocytosis |
| title_short |
The role of substrate mechanics in nanotoxicity mediated by endocytosis |
| title_full |
The role of substrate mechanics in nanotoxicity mediated by endocytosis |
| title_fullStr |
The role of substrate mechanics in nanotoxicity mediated by endocytosis |
| title_full_unstemmed |
The role of substrate mechanics in nanotoxicity mediated by endocytosis |
| title_sort |
role of substrate mechanics in nanotoxicity mediated by endocytosis |
| publisher |
Wright State University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=wright1516127191655069 |
| work_keys_str_mv |
AT boehmrobertc theroleofsubstratemechanicsinnanotoxicitymediatedbyendocytosis AT boehmrobertc roleofsubstratemechanicsinnanotoxicitymediatedbyendocytosis |
| _version_ |
1719453383885062144 |