Effect of Rat Strain Stereotactic Coordinates on Infarct Volume

Effect of Rat Strain Stereotactic Coordinates on Infarct Volume

Bibliographic Details
Main Author: Sanghvi, Saagar K.
Language:English
Published: Wright State University / OhioLINK 2013
Subjects:
Neurosciences
Neurology
Neurobiology
Physiology
Anatomy and Physiology
stroke
infarct volume
rat strain
Montoya Staircase
ischemia
fluoxetine
simvastatin
sub-ventricular zone
stereotaxic coordinates
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=wright1364484571
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-wright13644845712021-08-03T05:21:28Z Effect of Rat Strain Stereotactic Coordinates on Infarct Volume Sanghvi, Saagar K. Neurosciences Neurology Neurobiology Physiology Anatomy and Physiology stroke infarct volume rat strain Montoya Staircase ischemia fluoxetine simvastatin sub-ventricular zone stereotaxic coordinates Ischemic stroke makes up 87% of all hospital-admitted stroke cases annually; the primary treatment for these cases is intravenous administration of tPA within a 3.5 hour window from stroke onset. A long-term delayed ischemic stroke treatment proposed by this study was a combination of the pharmaceuticals Fluoxetine (SSRI), Simvastatin (statin), and ascorbic acid (Vitamin C). 51 adult rat subjects (10-12 months of age; 44 Sprague Dawley, 7 Long Evans) were given a combination of the drugs for 31 days. Drugs were given through voluntary oral administration via sugar cookie-dough balls to reduce inhibition of neurogenesis through stress-related glucocorticoid production. Drug combinations were as follows: FSA - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin and 20 mg/kg ascorbic acid; FS - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin; and the vehicle control. Endothelin-induced cortical stroke was administered using 2 different set of coordinates relative to bregma: Group 1 - (AP: 0.0 mm, ML: -2.5 mm) and (AP +1.5 mm; ML: -2.5 mm); Group 2 - (AP: 0.0 mm, ML: -2.5mm) and (AP +2.3; ML -2.5 mm). To analyze functional deficit, rats were subject to Montoya Staircase functional test once pre-stroke and twice post-stroke, and the Forelimb Asymmetry functional test once pre-stroke and thrice post-stroke. Results showed that Long Evans rats sustain a significantly larger infarct volume compared to Sprague Dawley rats using Group 2 coordinates; Group 1 cortical injection coordinates produced a larger infarct than Group 2 coordinates in FSA Sprague Dawley rats; drug treatment showed no effect on total infarct volume, however, this may be attributed to use of generic fluoxetine in Group 2 Sprague Dawley rats. 2013-05-01 English text Wright State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=wright1364484571 http://rave.ohiolink.edu/etdc/view?acc_num=wright1364484571 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Neurosciences
Neurology
Neurobiology
Physiology
Anatomy and Physiology
stroke
infarct volume
rat strain
Montoya Staircase
ischemia
fluoxetine
simvastatin
sub-ventricular zone
stereotaxic coordinates
spellingShingle Neurosciences
Neurology
Neurobiology
Physiology
Anatomy and Physiology
stroke
infarct volume
rat strain
Montoya Staircase
ischemia
fluoxetine
simvastatin
sub-ventricular zone
stereotaxic coordinates
Sanghvi, Saagar K.
Effect of Rat Strain Stereotactic Coordinates on Infarct Volume
author Sanghvi, Saagar K.
author_facet Sanghvi, Saagar K.
author_sort Sanghvi, Saagar K.
title Effect of Rat Strain Stereotactic Coordinates on Infarct Volume
title_short Effect of Rat Strain Stereotactic Coordinates on Infarct Volume
title_full Effect of Rat Strain Stereotactic Coordinates on Infarct Volume
title_fullStr Effect of Rat Strain Stereotactic Coordinates on Infarct Volume
title_full_unstemmed Effect of Rat Strain Stereotactic Coordinates on Infarct Volume
title_sort effect of rat strain stereotactic coordinates on infarct volume
publisher Wright State University / OhioLINK
publishDate 2013
url http://rave.ohiolink.edu/etdc/view?acc_num=wright1364484571
work_keys_str_mv AT sanghvisaagark effectofratstrainstereotacticcoordinatesoninfarctvolume
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