Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies

Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies

Bibliographic Details
Main Author: Alhejely, Amani Saud
Language:English
Published: Wright State University / OhioLINK 2011
Subjects:
Immunology
Microbiology
HIV-1 mutation rate
amino acid
Reverse Transcriptase
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=wright1309968759
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-wright13099687592021-08-03T06:17:26Z Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies Alhejely, Amani Saud Immunology Microbiology HIV-1 mutation rate amino acid Reverse Transcriptase As research has shown, a high mutation rate occursduring the reverse transcription RT process because HIVreverse transcriptase fails to correct erroneouslyincorporated nucleotides during the reverse transcriptionprocess. Based on various research articles, a series ofamino acid substitutions created in RT were collected tofind out their influence on virus mutation frequencies. Twotables were used; one for amino acid substitutions createdin RT that increased the HIV-1 mutation rate and anotherfor amino acid substitutions created in RT that decreasedthe HIV-1 mutation rate. The tables show which amino acidsubstitutions created in RT decreased or increased themutation rate of HIV-1. With more amino acid substitutionsshowing a decrease in the mutation rate of HIV-1, thetables provide researchers with helpful information forfuture research. Additionally, the information resulted inthree new findings. First, When viewing single amino acidsidentified with drug resistance, most showed a decrease inthe frequency of the mutation rate the of HIV-1; however,if the specified amino acid is not identified with drug resistance most showed an increase in the HIV-1 mutationrate. Second, when HIV-1 has an increased mutation ratemultiple amino acids showed drug resistance. Finally, astructural rationale for the location of residuesimplicated in the resistance of nucleoside-analoginhibitors (Huang et al., 1998) showed that mutations thatdecrease and/or increase the mutation rate do not clustertogether. Those specified mutations are all around theactive site in the crystal structure. Also, these aminoacids might be in the primer grip region or interact withthe incoming dNTP, and form the dNTP binding site orresidues in the fingers subdomain. 2011-07-07 English text Wright State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=wright1309968759 http://rave.ohiolink.edu/etdc/view?acc_num=wright1309968759 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Immunology
Microbiology
HIV-1 mutation rate
amino acid
Reverse Transcriptase
spellingShingle Immunology
Microbiology
HIV-1 mutation rate
amino acid
Reverse Transcriptase
Alhejely, Amani Saud
Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies
author Alhejely, Amani Saud
author_facet Alhejely, Amani Saud
author_sort Alhejely, Amani Saud
title Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies
title_short Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies
title_full Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies
title_fullStr Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies
title_full_unstemmed Amino acid substitutions created in Reverse Transcriptase and their influence on HIV-1 mutation frequencies
title_sort amino acid substitutions created in reverse transcriptase and their influence on hiv-1 mutation frequencies
publisher Wright State University / OhioLINK
publishDate 2011
url http://rave.ohiolink.edu/etdc/view?acc_num=wright1309968759
work_keys_str_mv AT alhejelyamanisaud aminoacidsubstitutionscreatedinreversetranscriptaseandtheirinfluenceonhiv1mutationfrequencies
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