Inhibition of Cell Invasion by Targeting PLD
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Wright State University / OhioLINK
2010
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=wright1292449970 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-wright12924499702021-08-03T06:17:13Z Inhibition of Cell Invasion by Targeting PLD Farkaly, Terry C. Molecular Biology PLD cell invasion inhibition lipase activity mtln3 Phospholipase D (PLD) is a crucial signaling enzyme involved in many cellularprocesses. The catalytic activity of PLD is essential for the production of Phosphatidic Acid(PA), a critical second messenger in cell signaling cascades downstream. Using the highlyinvasive rat mammary adenocarcinoma cell line mTLn3 as a metastatic model, we investigatedthe proficiency of these cells to invade using matrigels that mimic the basement membrane of theextracellular matrix (ECM), their activity through PLD enzymatic assays, as well as the potencyof our potential inhibitors to inhibit PLD-mediated cell invasion and lipase activity.This study reveals that PLD-mediated cell invasion is dependent on protein-proteininteractions with other cell signaling molecules such as Grb2 and Rac2 and their effect on lipaseactivity. Regulation of PLD2 activity by phosphorylation on tyrosine residue sites within itsPhox domain are examined; in which we elucidate the effects of specific kinases EGFR, Jak3and Src on cell invasion and enzymatic activity. Based off this approach, a therapeutic resolutionwill be proposed to diminish cell invasion in metastasis. 2010-12-16 English text Wright State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=wright1292449970 http://rave.ohiolink.edu/etdc/view?acc_num=wright1292449970 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Molecular Biology PLD cell invasion inhibition lipase activity mtln3 |
| spellingShingle |
Molecular Biology PLD cell invasion inhibition lipase activity mtln3 Farkaly, Terry C. Inhibition of Cell Invasion by Targeting PLD |
| author |
Farkaly, Terry C. |
| author_facet |
Farkaly, Terry C. |
| author_sort |
Farkaly, Terry C. |
| title |
Inhibition of Cell Invasion by Targeting PLD |
| title_short |
Inhibition of Cell Invasion by Targeting PLD |
| title_full |
Inhibition of Cell Invasion by Targeting PLD |
| title_fullStr |
Inhibition of Cell Invasion by Targeting PLD |
| title_full_unstemmed |
Inhibition of Cell Invasion by Targeting PLD |
| title_sort |
inhibition of cell invasion by targeting pld |
| publisher |
Wright State University / OhioLINK |
| publishDate |
2010 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=wright1292449970 |
| work_keys_str_mv |
AT farkalyterryc inhibitionofcellinvasionbytargetingpld |
| _version_ |
1719434093678034944 |