Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis

Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis

Bibliographic Details
Main Author: Marin, Brianna
Language:English
Published: Walsh University Honors Theses / OhioLINK 2019
Subjects:
Biology
Molecular Biology
Pharmacology
Virology
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1555516450619539
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-walshhonors15555164506195392021-08-03T07:10:29Z Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis Marin, Brianna Biology Molecular Biology Pharmacology Virology Herpes simplex virus type-1 (HSV-1) is a highly contagious double-stranded DNA virusthat impacts at least one-third of the worlds’ population. HSV-1, or oral herpes, typically causespainful facial-oral lesions, and can enter into a period of asymptomatic latency, makingtherapeutic options difficult to generate. Heat shock proteins are universal proteins expressedduring times of stress to cells, during which they facilitate protein assembly and regulate proteinswithin a host cell. Viral infection causes an increase in abundance of HSP’s, which the virusutilizes to ensure the proper assembly and conformation of viral proteins. Previous results in thislaboratory showed that HSP70 increased in both transcription and translation during viralinfection. Using Western immunoblotting, Byelene (2014) detected an increase in HSP70production two hours post-HSV infection until eight hours post-infection during a twelve hourtime-course analysis. Using real-time PCR, Kardohely (2016) noted an increase in geneexpression of hsp70 between hours five and seven post-infection. Research suggests thatinhibition of heat shock protein synthesis may lead to decreased viral replication and serve as apotential anti-viral therapy. The objective of this study was to identify the critical time during theHSV-1 replication cycle during which application of KNK437, a HSP70 inhibitor, had thegreatest influence on inhibiting viral proliferation in infected Vero cells. Following a time-coursetreatment, 0.1% of KNK437 was added to Vero cells hourly until hour ten post-infection. Virusquantification was conducted utilizing plaque titration. The results of this study illustrate thatKNK437 worked to decreased viral replication 99.6% during the time period one to three hoursafter viral introduction. These results suggest that HSP’s play a role early in virus replication andfurther work is required to demonstrate the efficacy of using HSP70 inhibitors in treating HSVinfections. 2019-04-30 English text Walsh University Honors Theses / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1555516450619539 http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1555516450619539 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biology
Molecular Biology
Pharmacology
Virology
spellingShingle Biology
Molecular Biology
Pharmacology
Virology
Marin, Brianna
Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis
author Marin, Brianna
author_facet Marin, Brianna
author_sort Marin, Brianna
title Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis
title_short Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis
title_full Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis
title_fullStr Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis
title_full_unstemmed Determining the antiviral effect of HSP70 inhibitor, KNK437, by a time-dependent analysis
title_sort determining the antiviral effect of hsp70 inhibitor, knk437, by a time-dependent analysis
publisher Walsh University Honors Theses / OhioLINK
publishDate 2019
url http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1555516450619539
work_keys_str_mv AT marinbrianna determiningtheantiviraleffectofhsp70inhibitorknk437byatimedependentanalysis
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