Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor
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Walsh University Honors Theses / OhioLINK
2016
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1461684985 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-walshhonors14616849852021-08-03T06:36:12Z Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor Thomas, Matthew Medicine Preeclampsia is a potentially fatal condition that affects maternal and fetal healthat the utero-placental barrier. Researchers propose that preeclampsia is a result of animproper balance of angiogenic factors and their receptors, specifically a decreasedamount of free vascular endothelial growth factor (VEGF) available to bind to membranebound VEGF receptor. This imbalance is believed to impair spiral artery remodeling inthe decidua, which results in maternal hypertension. The primary purpose of this studywas to evaluate the effects of L-Tyrosine Polyphosphate (LTP) nanoparticles injectionscontaining exogenous DNA which result in an up regulation of membrane bound VEGFreceptor (VEGF-R2) had on the maternal and fetal health, specifically the cerebral arteriesof the mother. We used the reduced uterine perfusion pressure (RUPP) model to inducepreeclamptic-like symptoms in pregnant Sprauge-Dawley rats. The RUPP model reducedblood flow to the uterus by partially occluding the descending abdominal aorta anduterine-ovarian arcades, and had been proven to illicit physiologic changes similar topreeclampsia including altered vessel tone. The control group (Sham) underwentsurgery, but did not receive the occluding clips, thus did not develop preeclamptic-likesymptoms. Additionally, at the time of surgery the rats were either injected with LTPnanoparticlesor not, which resulted in four groups Rupp, Rupp-Treated, Sham, andSham-Treated. The rats were euthanized at day 20 of pregnancy, and their posteriorcerebral arteries (PCA) were removed. The vessels were hung on an isobaric arteriographand subjected to an array of pressures to assess the vessels’ myogenic tones. We foundthat VEGF-R2 injections via nanoparticles significantly reduced maternal hypertension (p< 0.5) significantly improved uterine artery myogenic responsiveness (p < 0.5),significantly increased fetal weight (p < 0.5), and most likely reduces myogenic tone ofthe posterior cerebral arteries. Western Blotting was also performed to compare theamount of phosphorylated VEGF-R2 present in the in the various groups. The results ofthe Western Blotting were inconclusive. The results suggest that VEGF-R2 injections viananoparticles did improve spiral artery angiogenesis improving maternal health. 2016-04-26 English text Walsh University Honors Theses / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1461684985 http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1461684985 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Medicine |
| spellingShingle |
Medicine Thomas, Matthew Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor |
| author |
Thomas, Matthew |
| author_facet |
Thomas, Matthew |
| author_sort |
Thomas, Matthew |
| title |
Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor |
| title_short |
Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor |
| title_full |
Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor |
| title_fullStr |
Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor |
| title_full_unstemmed |
Effects of Reduced Uterine Perfusion Pressure on Cerebral Artery Tone inPregnant Rats Treated with Nanoparticles Containing VEGF Receptor |
| title_sort |
effects of reduced uterine perfusion pressure on cerebral artery tone inpregnant rats treated with nanoparticles containing vegf receptor |
| publisher |
Walsh University Honors Theses / OhioLINK |
| publishDate |
2016 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=walshhonors1461684985 |
| work_keys_str_mv |
AT thomasmatthew effectsofreduceduterineperfusionpressureoncerebralarterytoneinpregnantratstreatedwithnanoparticlescontainingvegfreceptor |
| _version_ |
1719440310949380096 |