Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele
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| Language: | English |
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University of Cincinnati / OhioLINK
2018
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535635832679162 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin1535635832679162 |
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oai_dc |
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NDLTD |
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English |
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Biomedical Research amniotic fluid polymer blends in-vitro degradation in-vivo biocompatibility surgical patch |
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Biomedical Research amniotic fluid polymer blends in-vitro degradation in-vivo biocompatibility surgical patch Tatu, Rigwed R. Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele |
| author |
Tatu, Rigwed R. |
| author_facet |
Tatu, Rigwed R. |
| author_sort |
Tatu, Rigwed R. |
| title |
Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele |
| title_short |
Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele |
| title_full |
Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele |
| title_fullStr |
Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele |
| title_full_unstemmed |
Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele |
| title_sort |
development and characterization of apoly (l-lactic acid)/ poly (e-caprolactone) self-expanding patch forfetoscopic repair of myelomeningocele |
| publisher |
University of Cincinnati / OhioLINK |
| publishDate |
2018 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535635832679162 |
| work_keys_str_mv |
AT taturigwedr developmentandcharacterizationofapolyllacticacidpolyecaprolactoneselfexpandingpatchforfetoscopicrepairofmyelomeningocele |
| _version_ |
1719454621764681728 |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-ucin15356358326791622021-08-03T07:08:29Z Development and Characterization of aPoly (l-lactic acid)/ Poly (e-caprolactone) Self-Expanding Patch forFetoscopic Repair of Myelomeningocele Tatu, Rigwed R. Biomedical Research amniotic fluid polymer blends in-vitro degradation in-vivo biocompatibility surgical patch Myelomeningocele (MMC) is a neurologic defect characterized by failure of neural tube closure in the spinal column. This leads to cerebrospinal fluid leakage or contact with amniotic fluid, which can translate into sexual dysfunctions and paralysis after birth. The recently developed minimally-invasive technique for MMC repair is called fetoscopy, which involves a surgical patch, expanded for defect coverage on the fetus’ back. Currently used inert patches do not degrade after implantation, necessitating a post-natal removal surgery, while collagen-based patches employed in are associated with poor mechanical integrity. Also, these patches are not tailored for fetoscopic MMC repair, and their response in fetal environment is unexplored. Deployment and expansion of coiled patch using surgical tools at defect site is time-consuming and cumbersome. Some of these existing patches have mesh-like structure for tissue in-growth, which makes their barrier properties debatable. Upon implantation at defect site, the patch encounters amniotic fluid and body fluids, as well as fluid forces due to fetal movement in the womb. This necessitates analysis of biodegradability and mechanical response of the patch for its adaptability in fetoscopic MMC repair. Taking the above requirements into consideration, we designed a patch comprising a blend of poly (L-lactic acid) (PLA) and poly (ε-caprolactone) (PCL), both polymers approved by the U.S. Food and Drug Administration for hard and soft tissue repair in spine. Different PLA-PCL formulations were characterized for surface and thermal properties, and the ideal formulation was chosen as our designed patch based on aptitude for thermal expansion at in-vivo temperature (37°C). This will enable self-expansion of the coiled patch at defect site, saving time and reducing difficulty level of surgery. The designed patch was characterized for barrier properties to ensure its watertight nature, and for biocompatibility after exposure to human foreskin fibroblasts, to confirm absence of solvent traces from fabrication. Bio-degradation of designed patch was studied in simulated fetal environment of human amniotic fluid. Patch strips were immersed in human amniotic fluid and subjected to a simulated fetal environment. An additional set of patch strips was immersed in phosphate-buffered saline as a time-paired control. Changes in weight, surface roughness, functional groups, crystallinity and mechanical properties were investigated at 4, 8, 12 and 16-week time points. Our principal findings indicate the progress of hydrolytic degradation in amniotic fluid, with brittle behavior observed at 16 weeks. However, no deterioration of mechanical and barrier properties was observed for application in fetoscopic MMC repair. We analyzed the biocompatibility of our patch in two in-vivo animal models. In the first one, we demonstrated the encapsulation of the patch with no immune reaction on subcutaneous implantation and in the second one we demonstrated compatibility of the patch as a dural substitute via laminectomy. The patches when in contact with the spinal cord as a dural substitute will not induce any adverse effect such as scar formation or tethering cord and functions of the spinal cord. The designed patch successfully fulfilled all requirements and serves as a standalone system to tackle impending hurdles of MMC repair. 2018-10-30 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535635832679162 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535635832679162 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |